Composite

Part:BBa_K133020:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CMV-CF213-multiHP-CF215-RGD-Histop (term.)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1963
    Illegal BamHI site found at 2474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2757


Design Notes

The part was cloned into pSB1.AK3 with terminator for use in bacterial cells (surface expression).

Source

multiplied with PCR from plasmid or ordered from GeneArt

References